In the past several years, the advent of recombinant DNA technology was seen as a revolution of biotechnology. Recombinant DNA, also known as rDNA, is an artificial type of DNA which formed by combining two or more sequences and strands of DNA that would not occur together usually. rDNA is sometimes called genetic engineering.
The applications of recombinant DNA technology are widely used and increasing recently for various kinds of purposes for example, expand the potential of microorganisms, to produce natural proteins manufacture of vaccines or enzymes. Recombinant DNA is made by three common ways which included the transformation, phage introduction and non-bacterial transformation. Transformation is the step of inserting the interest DNA into a vector and then cut that piece of DNA with a restricted enzyme and then insert the DNA into the vector with DNA Ligase. After that the vector is inserted to a host cell like E. coli. The phage introduction is the process of transfection, which can be said as an equivalent of transformation but instead of using bacteria, a phage is used. This process uses lambda or other phages to produce phage plaques which contain recombinant. In non-bacterial transformation, a process which very is similar to transformation never use bacterial for the host. In microinjection, the DNA is injected directly into the nucleus of the cell being transformed while in biolistics, the host cells are bombarded with high velocity of microprojectiles. This technique was discovered by Peter Lobban and A. Dale Kaiser in Stanford University.
The applications of recombinant DNA technology are widely used and increasing recently for various kinds of purposes for example, expand the potential of microorganisms, to produce natural proteins manufacture of vaccines or enzymes. Recombinant DNA is made by three common ways which included the transformation, phage introduction and non-bacterial transformation. Transformation is the step of inserting the interest DNA into a vector and then cut that piece of DNA with a restricted enzyme and then insert the DNA into the vector with DNA Ligase. After that the vector is inserted to a host cell like E. coli. The phage introduction is the process of transfection, which can be said as an equivalent of transformation but instead of using bacteria, a phage is used. This process uses lambda or other phages to produce phage plaques which contain recombinant. In non-bacterial transformation, a process which very is similar to transformation never use bacterial for the host. In microinjection, the DNA is injected directly into the nucleus of the cell being transformed while in biolistics, the host cells are bombarded with high velocity of microprojectiles. This technique was discovered by Peter Lobban and A. Dale Kaiser in Stanford University.
