Erwin Chargaff, an American biochemist who who emigrated to the United States during the Nazi era . He discovered 2 rules that help to study the structure of double helix DNA. According to Chargaff's rule:
- DNA has equal number of adenine and thymine residues (A=T) and equal numbers of guanine and cytosine residues(G=C)
- This is the Chargaff”s rule of molar equivalence between the purines and pyrimidines in DNA structure.
- The double helical structure of DNA derives its strength from Chargaff’s rule.
- Single stranded DNA and RNAs which are usually single stranded do not obey the rule.
- Two possible helical forms of DNA are mirror images of each other.
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